The SARS-CoV ferret model in an infection-challenge study.
Identifieur interne : 002F96 ( Main/Exploration ); précédent : 002F95; suivant : 002F97The SARS-CoV ferret model in an infection-challenge study.
Auteurs : Yong-Kyu Chu [États-Unis] ; Georgia D. Ali ; Fuli Jia ; Qianjun Li ; David Kelvin ; Ronald C. Couch ; Kevin S. Harrod ; Julie A. Hutt ; Cheryl Cameron ; Susan R. Weiss ; Colleen B. JonssonSource :
- Virology [ 0042-6822 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Fosse nasale (virologie), Furets, Modèles animaux de maladie humaine, Mâle, Numération des leucocytes, Numération des lymphocytes, Pharynx (virologie), Poumon (anatomopathologie), Poumon (virologie), Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (physiopathologie), Syndrome respiratoire aigu sévère (virologie), Température du corps, Tests de neutralisation, Virus du SRAS (physiologie).
- MESH :
- anatomopathologie : Poumon, Syndrome respiratoire aigu sévère.
- immunologie : Syndrome respiratoire aigu sévère.
- physiologie : Virus du SRAS.
- physiopathologie : Syndrome respiratoire aigu sévère.
- sang : Anticorps antiviraux.
- virologie : Fosse nasale, Pharynx, Poumon, Syndrome respiratoire aigu sévère.
- Animaux, Furets, Modèles animaux de maladie humaine, Mâle, Numération des leucocytes, Numération des lymphocytes, Température du corps, Tests de neutralisation.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Body Temperature, Disease Models, Animal, Ferrets, Leukocyte Count, Lung (pathology), Lung (virology), Lymphocyte Count, Male, Nasal Cavity (virology), Neutralization Tests, Pharynx (virology), SARS Virus (physiology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (pathology), Severe Acute Respiratory Syndrome (physiopathology), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , blood : Antibodies, Viral.
- immunology : Severe Acute Respiratory Syndrome.
- pathology : Lung, Severe Acute Respiratory Syndrome.
- physiology : SARS Virus.
- physiopathology : Severe Acute Respiratory Syndrome.
- virology : Lung, Nasal Cavity, Pharynx, Severe Acute Respiratory Syndrome.
- Animals, Body Temperature, Disease Models, Animal, Ferrets, Leukocyte Count, Lymphocyte Count, Male, Neutralization Tests.
Abstract
Phase I human clinical studies involving therapeutics for emerging and biodefense pathogens with low incidence, such as the severe acute respiratory syndrome coronavirus (SARS-CoV), requires at a minimum preclinical evaluation of efficacy in two well-characterized and robust animal models. Thus, a ferret SARS-CoV model was evaluated over a period of 58 days following extensive optimization and characterization of the model in order to validate clinical, histopathological, virological and immunological endpoints. Ferrets that were infected intranasally with 10(3) TCID50 SARS-CoV showed higher body temperature (2-6 d.p.i.), sneezing (5-10 d.p.i.), lesions (5-7 d.p.i.) and decreased WBC/lymphocytes (2-5 d.p.i.). SARS-CoV was detected up to 7 d.p.i. in various tissues and excreta, while neutralizing antibody titers rose at 7 d.p.i. and peaked at 14 d.p.i. At 29 d.p.i., one group was challenged with 10(3) TCID50 SARS-CoV, and an anamnestic response in neutralizing antibodies was evident with no detectable virus. This study supports the validity of the ferret model for use in evaluating efficacy of potential therapeutics to treat SARS.
DOI: 10.1016/j.virol.2007.12.032
PubMed: 18234270
Affiliations:
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Jonsson</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Body Temperature</term><term>Disease Models, Animal</term><term>Ferrets</term><term>Leukocyte Count</term><term>Lung (pathology)</term><term>Lung (virology)</term><term>Lymphocyte Count</term><term>Male</term><term>Nasal Cavity (virology)</term><term>Neutralization Tests</term><term>Pharynx (virology)</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (pathology)</term><term>Severe Acute Respiratory Syndrome (physiopathology)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Fosse nasale (virologie)</term><term>Furets</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Numération des leucocytes</term><term>Numération des lymphocytes</term><term>Pharynx (virologie)</term><term>Poumon (anatomopathologie)</term><term>Poumon (virologie)</term><term>Syndrome respiratoire aigu sévère (anatomopathologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (physiopathologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Température du corps</term><term>Tests de neutralisation</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Lung</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fosse nasale</term><term>Pharynx</term><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term><term>Nasal Cavity</term><term>Pharynx</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Body Temperature</term><term>Disease Models, Animal</term><term>Ferrets</term><term>Leukocyte Count</term><term>Lymphocyte Count</term><term>Male</term><term>Neutralization Tests</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Furets</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Numération des leucocytes</term><term>Numération des lymphocytes</term><term>Température du corps</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phase I human clinical studies involving therapeutics for emerging and biodefense pathogens with low incidence, such as the severe acute respiratory syndrome coronavirus (SARS-CoV), requires at a minimum preclinical evaluation of efficacy in two well-characterized and robust animal models. Thus, a ferret SARS-CoV model was evaluated over a period of 58 days following extensive optimization and characterization of the model in order to validate clinical, histopathological, virological and immunological endpoints. Ferrets that were infected intranasally with 10(3) TCID50 SARS-CoV showed higher body temperature (2-6 d.p.i.), sneezing (5-10 d.p.i.), lesions (5-7 d.p.i.) and decreased WBC/lymphocytes (2-5 d.p.i.). SARS-CoV was detected up to 7 d.p.i. in various tissues and excreta, while neutralizing antibody titers rose at 7 d.p.i. and peaked at 14 d.p.i. At 29 d.p.i., one group was challenged with 10(3) TCID50 SARS-CoV, and an anamnestic response in neutralizing antibodies was evident with no detectable virus. This study supports the validity of the ferret model for use in evaluating efficacy of potential therapeutics to treat SARS.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Alabama</li></region></list><tree><noCountry><name sortKey="Ali, Georgia D" sort="Ali, Georgia D" uniqKey="Ali G" first="Georgia D" last="Ali">Georgia D. Ali</name><name sortKey="Cameron, Cheryl" sort="Cameron, Cheryl" uniqKey="Cameron C" first="Cheryl" last="Cameron">Cheryl Cameron</name><name sortKey="Couch, Ronald C" sort="Couch, Ronald C" uniqKey="Couch R" first="Ronald C" last="Couch">Ronald C. Couch</name><name sortKey="Harrod, Kevin S" sort="Harrod, Kevin S" uniqKey="Harrod K" first="Kevin S" last="Harrod">Kevin S. Harrod</name><name sortKey="Hutt, Julie A" sort="Hutt, Julie A" uniqKey="Hutt J" first="Julie A" last="Hutt">Julie A. Hutt</name><name sortKey="Jia, Fuli" sort="Jia, Fuli" uniqKey="Jia F" first="Fuli" last="Jia">Fuli Jia</name><name sortKey="Jonsson, Colleen B" sort="Jonsson, Colleen B" uniqKey="Jonsson C" first="Colleen B" last="Jonsson">Colleen B. Jonsson</name><name sortKey="Kelvin, David" sort="Kelvin, David" uniqKey="Kelvin D" first="David" last="Kelvin">David Kelvin</name><name sortKey="Li, Qianjun" sort="Li, Qianjun" uniqKey="Li Q" first="Qianjun" last="Li">Qianjun Li</name><name sortKey="Weiss, Susan R" sort="Weiss, Susan R" uniqKey="Weiss S" first="Susan R" last="Weiss">Susan R. Weiss</name></noCountry><country name="États-Unis"><region name="Alabama"><name sortKey="Chu, Yong Kyu" sort="Chu, Yong Kyu" uniqKey="Chu Y" first="Yong-Kyu" last="Chu">Yong-Kyu Chu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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